Educational Resources

Biotechnology Fundamentals - Lectures - Coming Soon!

 

 

 

Biotechnology Laboratory Modules

Laboratory 1 : Microbiology Basics & Protein Expression 1. 

Key Informational Videos

1. Pouring Agar Plates 

2. Isolation Streaking for Single Colonies

3. Basics of Protein Expression in E. coli 

4. Overview of Proteins we are expressing - Taq

5. Overview of Proteins we are expressing - Phusion

6. Overview of Proteins we are expressing - cas9

PreLab Quiz 

Lab Checklist

Lab Protocol 

Lab Data Sheet (None for Lab 1)

 

Laboratory 2 : Protein Expression 2, Analyzing Expression Levels, Cell Harvest

Key Informational Videos

1. Composition of E. coli (Biomass) 

2. SDS PAGE

3. Image J 

4. Overview of Fermentation

5. Overview of Centrifugation & Centrifuge Design

PreLab Quiz 

Lab Checklist

Lab Protocol 

Lab Data Sheet 

Lab Report 1 Template 

 

Laboratory 3 : Protein Expression 3, Cell Lysis & Basics of DSP

Key Informational Videos

1. Overview of Types of Cell Lysis 

2. Overview of Analytical Methods

3. Overview of Bradford Assay

4. Overview of Downstream Recovery 

PreLab Quiz 

Lab Checklist

Lab Protocol 

Lab Data Sheet 

 

Laboratory 4 : Downstream Purification 2: His Tag Purification 

Key Informational Videos

1. Overview of His Tag Purification 

PreLab Quiz 

Lab Checklist

Lab Protocol 

Lab Data Sheet 

 

Laboratory 5 : Downstream Purification 3: Protein Precipitation 

Key Informational Videos

1. Overview of Heat Denaturation of Proteins 

PreLab Quiz 

Lab Checklist

Lab Protocol 

Lab Data Sheet 

Lab Report 2 Template 

 

 

Laboratory 6 : Downstream Purification 4: Chromatography 

Key Informational Videos

1. Overview of Types of Chromatography

2. Types of Chromatography use for Proteins (Size, Charge, Affinity)

3. Overview of Protein Size and pI

4. Modelling Chromatography 

5. Chromatography Design 

PreLab Quiz 

Lab Checklist

Lab Protocol 

Lab Data Sheet 

 

Laboratory 7 : Downstream Purification 5: Tangential Flow Filtration (TFF)

Key Informational Videos

1. Overview of GFP Purification by TFF (six 3 minute videos) 

2. Modeling Filtration  

3. Filtration Design 

PreLab Quiz 

Lab Checklist

Lab Protocol 

Lab Data Sheet 

Lab Report 3 Template  

 

Laboratory 8 : Protein Quality Control & Enzyme Kinetics 

Key Informational Videos

1. Overview of qPCR

2. Overview of Michaelis Menten 

3. Overview of cas9 digestion 

4. Overview of Quality 

PreLab Quiz 

Lab Checklist

Lab Protocol 

Lab Data Sheet 

Lab Report 4 Template