Identification of a 21 amino acid peptide conferring 3-hydroxypropionic acid stress-tolerance to Escherichia coli.

TitleIdentification of a 21 amino acid peptide conferring 3-hydroxypropionic acid stress-tolerance to Escherichia coli.
Publication TypeJournal Article
Year of Publication2012
AuthorsWarnecke, TE, Lynch, MD, Lipscomb, ML, and Gill, RT
JournalBiotechnology & Bioengineering
Volume109
Issue5
Start Page1347
Pagination1347 - 1352
Date Published05/2012
Abstract

We report the identification of a novel small open reading frame in Escherichia coli. The sORF (called iroK) encodes a 21 amino cid peptide, which when translated confers a 133% (ca. 20 g/L) increase in resistance to 3-hydroxypropionic acid. We show that iroK conferred tolerance is additive to previously identified tolerance mechanisms involving relief of inhibited metabolism, yet does not involve altered 3-HP transport. This result demonstrates the continued surprises that microbial genomes hold and emphasize the importance of comprehensive discovery methods in future strain and metabolic engineering efforts.

DOI10.1002/bit.24398
Short TitleBiotechnology & Bioengineering