|Title||Identification of a 21 amino acid peptide conferring 3-hydroxypropionic acid stress-tolerance to Escherichia coli.|
|Publication Type||Journal Article|
|Year of Publication||2012|
|Authors||Warnecke, TE, Lynch, MD, Lipscomb, ML, and Gill, RT|
|Journal||Biotechnology & Bioengineering|
|Pagination||1347 - 1352|
We report the identification of a novel small open reading frame in Escherichia coli. The sORF (called iroK) encodes a 21 amino cid peptide, which when translated confers a 133% (ca. 20 g/L) increase in resistance to 3-hydroxypropionic acid. We show that iroK conferred tolerance is additive to previously identified tolerance mechanisms involving relief of inhibited metabolism, yet does not involve altered 3-HP transport. This result demonstrates the continued surprises that microbial genomes hold and emphasize the importance of comprehensive discovery methods in future strain and metabolic engineering efforts.
|Short Title||Biotechnology & Bioengineering|