Broad-host-range vectors for protein expression across gram negative hosts.

TitleBroad-host-range vectors for protein expression across gram negative hosts.
Publication TypeJournal Article
Year of Publication2010
AuthorsJE Prior, MD Lynch, and RT Gill
JournalBiotechnology & Bioengineering
Volume106
Issue2
Start Page326
Pagination326 - 332
Date Published06/2010
Abstract

Expression of recombinant proteins across a range of different host organisms is often necessary in metabolic engineering applications. Doing so can be facilitated by the use of vectors having origins of replication with a broad-host-range, the option for antibiotic resistance cassettes that are compatible with a particular host, in addition to sequences allowing for the effective transcription and translation of target proteins. We have created a modular set of broad-host-range expression vectors for protein expression in Gram negative bacteria. These vectors use the broad-host-range pBBR1 replicon as well as the arabinose-inducible P(BAD) promoter and are available with six different antibiotic resistance cassettes. We have demonstrated the use of these vectors in Escherichia coli, Pseudomonas putida, and Burkholderia cepacia.

DOI10.1002/bit.22695
Short TitleBiotechnology & Bioengineering