|Title||Broad-host-range vectors for protein expression across gram negative hosts.|
|Publication Type||Journal Article|
|Year of Publication||2010|
|Authors||Prior, JE, Lynch, MD, and Gill, RT|
|Journal||Biotechnology & Bioengineering|
|Pagination||326 - 332|
Expression of recombinant proteins across a range of different host organisms is often necessary in metabolic engineering applications. Doing so can be facilitated by the use of vectors having origins of replication with a broad-host-range, the option for antibiotic resistance cassettes that are compatible with a particular host, in addition to sequences allowing for the effective transcription and translation of target proteins. We have created a modular set of broad-host-range expression vectors for protein expression in Gram negative bacteria. These vectors use the broad-host-range pBBR1 replicon as well as the arabinose-inducible P(BAD) promoter and are available with six different antibiotic resistance cassettes. We have demonstrated the use of these vectors in Escherichia coli, Pseudomonas putida, and Burkholderia cepacia.
|Short Title||Biotechnology & Bioengineering|